mouse melanoma cell line b16f10  (ATCC)

Journal: Redox Biology

Article Title: Combination of YAP inhibition and photodynamic therapy induces dual DNA damage and activates STING pathway to enhance immunotherapy in uveal melanoma

doi: 10.1016/j.redox.2025.103965

Figure Lengend Snippet: Activation of the cGAS-STING pathway by HANP/VP via YAP-inhibition and PDT in vitro. (A) The phosphorylation levels of representative proteins of the cGAS-STING pathway were analyzed using Western blot ( left ) and quantified ( right ). After HANP/VP treatment without laser irradiation, the pSTING/STING, pTBK1/TBK1, and pIRF3/IRF3 ratio were significantly increased compared to the untreated cells; the phosphorylation levels were further increased after laser irradiation, n = 3/group. (B) qPCR was used to detect IFN-β1 mRNA levels. Compared with the control group, HANP/VP without Laser treatment significantly increased IFN-β1 expression in B16F10 cells, while HANP/VP combined with laser irradiation further elevated IFN-β1 mRNA levels. When cells were pretreated with ethidium bromide (EB, 120 ng/mL), IFN-β1 mRNA levels decreased in the HANP/VP with Laser irradiation group. Following H151 treatment, IFN-β1 mRNA levels decreased to control group levels regardless of laser irradiation status. n = 3/group. Statistical significance was determined by one-way ANOVA (∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: The mouse melanoma cell line B16F10 was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Activation Assay, Inhibition, In Vitro, Phospho-proteomics, Western Blot, Irradiation, Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: Cell viability of MV3 and B16F10 cells treated with DSF/Cu. After MV3 and B16F10 cells were treated with DSF: 0–0.4 µM, Cu: 1 µM for 24 h. Cell viability was assessed using the MTT assay for MV3 ( A ) and B16F10 cells ( B ). Data were generated in triplicate and are presented as mean ± SD. The R 2 values of the fitted curves were 0.9683 for MV3 cells and 0.9820 for B16F10 cells.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: MTT Assay, Generated

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: DSF/Cu (Cu: 1 μM) + IR induces apoptosis of MV3 and B16F10 melanoma cells. After MV3 and B16F10 cells were treated with DSF: 0–0.2 µM, Cu: 1 µM and IR (0, 12 Gy) for 24 h, apoptotic cell rate (%) is determined by measuring Annexin V/7-AAD expression using flow cytometry ( A , B ). n = 3, * p < 0.05; ** p < 0.01; ns represents no significant difference.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: Expressing, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: Cell surface detection of CRT by MV3 and B16F10 cells following treatment with DSF/Cu + IR. After treatment with (DSF: 0–0.2 µM, Cu: 1 µM) and IR (0, 12 Gy) for 24 h, Cell surface CRT expression of MV3 and B16F10 cells is determined by flow cytometry ( A , B ). n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: Expressing, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: HMGB1 release from MV3 and B16F10 cells following treatment with DSF/Cu + IR. After treatment with DSF: 0–0.2 µM, Cu: 1 µM and IR (0, 12 Gy) for 24 h, HMGB1 release from MV3 and B16F10 cells was determined by ELISA ( A , B ). n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001; ns represents no significant difference.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: Intracellular ATP levels in MV3 and B16F10 cells following treatment with DSF/Cu + IR. After treatment with DSF: 0–0.2 µM, Cu: 1 µM and IR (0, 12 Gy) for 24 h, Intracellular ATP levels in MV3 and B16F10 cells were determined by flow cytometry ( A , B ). n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001; ns represents no significant difference.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: DSF/Cu + IR inhibits growth of B16F10 melanoma in vivo. B16F10 cells were implanted in C57BL/6 mice. Untreated group: mice were left untreated. DSF/Cu group: mice received intratumoral (i.t.) injections of 100 µL PBS containing DSF/Cu (DSF, 0.3 µM; Cu, 1 µM) on days 1 and 3. IR (12 Gy, single dose) group: tumor-localized irradiation (12 Gy) was delivered after the first intratumoral DSF in-jection in each treatment cycle, for a total of three cycles, with the remainder of the body shielded by lead. DSF/Cu + IR group: mice received intratumoral (i.t.) injections of 100 µL PBS containing DSF/Cu (DSF, 0.3 µM; Cu, 1 µM) on days 1 and 3. Tumor-localized IR (12 Gy) was delivered once on day 2. This treatment cycle was repeated three times at 7-day intervals ( A ). Tumor growth was monitored every two days. Tumor growth ( B ), Photos of isolated tumors from each group ( C ) were displayed. n ≥ 5, ** p < 0.01; *** p < 0.001.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: In Vivo, Irradiation, Isolation

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Combined with Irradiation Induces Immunogenic Cell Death in Melanoma

doi: 10.3390/ijms27020980

Figure Lengend Snippet: Flow cytometry analysis of tumor-infiltrating immune cells. Flow cytometry of tumor-infiltrating immune cells in B16F10 tumors following DSF/Cu and IR treatment. ( A ) The schematic of the gating strategy for CD8 + T cell, CD8 + T cell, DCs and MDSCs ( B – D ) The percentages of CD3 + T cells, CD3 + CD8 + T cells and CD3 + CD4 + T cells ( B ), DCs (CD11c + CD11b + cells ( C ) and MDSCs (CD11c + and GR-1 + cells) ( D ) in B16F10 tumors are displayed, respectively. No p value was reported because tumor cells from mice within each treatment group were pooled into a single sample, as tumors in the DSF/Cu + IR group were too small to analyze individually.

Article Snippet: Mouse melanoma B16F10 cell line, obtained from the American Type Culture Collection (ATCC), was maintained in DMEM (10-013-CV, Corning) with 10% FBS and 1% ( v / v ) penicillin–streptomycin.

Techniques: Flow Cytometry